Sampling methods for rumen microbial counts by Real-Time PCR techniques

Robust regression methods for real-time PCR

Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities

Molecular microbial ecology techniques are widely used to study the composition of the rumen microbiota and to increase understanding of the roles they play. Therefore, sampling and DNA extraction methods that result in adequate yields of microbial DNA that also accurately represents the microbial community are crucial. Fifteen different methods were used to extract DNA from cow and sheep rumen...

Sampling Techniques for Kernel Methods

We propose randomized techniques for speeding up Kernel Principal Component Analysis on three levels: sampling and quantization of the Gram matrix in training, randomized rounding in evaluating the kernel expansions, and random projections in evaluating the kernel itself. In all three cases, we give sharp bounds on the accuracy of the obtained approximations. Rather intriguingly, all three tech...

Guidelines for validation of qualitative real-time PCR methods

S. Broeders*, I. Huber, L. Grohmann, G. Berben, I. Taverniers, M. Mazzara, N. Roosens and D. Morisset Scientific Institute of Public Health (WIV-ISP), J. Wytsmanstraat 14, 1050 Brussels, Belgium (Tel.: D32 2 6425425; fax: D32 2 6425292; e-mails: [email protected]; nancy.roosens@ wiv-isp.be) Bavarian Health and Food Safety Authority (LGL), Veterin€arstraße 2, 85764 Oberschleißheim, Germ...

Statistical methods for efficiency adjusted real-time PCR quantification.

The statistical treatment for hypothesis testing using real-time PCR data is a challenge for quantification of gene expression. One has to consider two key factors in precise statistical analysis of real-time PCR data: a well-defined statistical model and the integration of amplification efficiency (AE) into the model. Previous publications in real-time PCR data analysis often fall short in int...